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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Polycistronic gene expression in Aspergillus niger

Fig. 3

Verification of mono-and polycistronic gene expression using Northern analysis. A fragment of the luciferase gene was used as a probe to detect luc mRNA in all strains. Results are exemplarily shown for the strains KEL and L in biological triplicates. Each sample was applied twice. rRNA bands are shown as a loading control. Note that a double band in the L sample is indicative for the two polyadenylation sites present in the trpC terminator as described earlier [27]. These two bands are not indistinguishable in the KEL sample due to the large size of the polycistronic mRNA. For details on the abbreviations the reader is referred to Table 1

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