Fig. 3From: Polycistronic gene expression in Aspergillus niger Verification of mono-and polycistronic gene expression using Northern analysis. A fragment of the luciferase gene was used as a probe to detect luc mRNA in all strains. Results are exemplarily shown for the strains KEL and L in biological triplicates. Each sample was applied twice. rRNA bands are shown as a loading control. Note that a double band in the L sample is indicative for the two polyadenylation sites present in the trpC terminator as described earlier [27]. These two bands are not indistinguishable in the KEL sample due to the large size of the polycistronic mRNA. For details on the abbreviations the reader is referred to Table 1 Back to article page