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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Use of the KlADH3 promoter for the quantitative production of the murine PDE5A isoforms in the yeast Kluyveromyces lactis

Fig. 6

Growth curves and viability of PDE5-transformed strains. a Growth curve of WT, pα-PDE5A1 and p3XFlagPDE5A1 transformed strains. Cultures were grown in YPG medium for 4 days and OD600 determined at time intervals. Each value is the average of three independent determinations with a standard deviation comprised between 4 and 13%. b Cell viability of cultures of A was determined at the end of 4 days of growth. Cell cultures were diluted to 108 cell/mL and the viability expressed as % of those forming colonies on YPD plates. c Western blot of PDE5A1 from the 2–4 days YPD-YPG-grown cultures extracts of the p3XFlagPDE5A1 strain. Each lane contains 4 μg of proteins. S. cerevisiae Pgk1 antibody was used as loading standard

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