Fig. 4From: Use of the KlADH3 promoter for the quantitative production of the murine PDE5A isoforms in the yeast Kluyveromyces lactis Native PAGE-migrating properties of cAMP/cGMP hydrolysing activities from WT and pα-PDE5A1 culture extracts. The activities of both extracts were determined in CPM (counts per minute) in the sequentially-sliced (1–20) migrated extracts shown in the Coomassie-stained gel insert. Extracts were obtained from cells grown in YPG medium for 4 daysBack to article page