Fig. 5

Hydrolysis of water-soluble wheat arabinoxylan by purified rPcAxe. 1, Substrate incubated without enzyme for 24 h (control); 2, hydrolysate treated with rPcAxe for 24 h; 3, arabinose standard; 4, xylose standard. Hydrolysis reactions were performed at 37 °C in buffer consisting of mixtures of 0.1 M citric acid and 0.2 M sodium hydrogen phosphate (pH 7.0) for 24 h with 1% (w/v) water-soluble wheat arabinoxylan loading. The amount of arabinose and xylose released was determined using HPLC. Values are the means and standard deviations of triplicate experiments