Table 1 Strains and plasmids used in this study
From: Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production
Strain/plasmid | Characteristic | Source |
|---|---|---|
Strain | ||
E. coli DH5ɑ | Clone host strain | Transgen |
C. glutamicum CICC 20190 | An l-arginine producing strain, ATCC 21493 | CICC |
C. crenatum MT-M4 | An l-arginine producing strain, derived from C. crenatum AS 1.542. | [23] |
C. glutamicum S9114 | Industrial strain for glutamate production | [14] |
Sorn1 | C. glutamicum S9114 with argF deletion | This study |
Sorn2 | Sorn1 with ncgl1221 deletion | This study |
Sorn3 | Sorn2 with argR deletion | This study |
Sorn4 | Sorn3 with putP deletion | This study |
Sorn5 | Sorn4 with RBS200 and A1G change in odhA | This study |
Sorn6 | Sorn4 with RBS400 and A1G change in odhA | This study |
Sorn7 | Sorn4 with RBS800 and A1G change in odhA | This study |
Sorn8 | Sorn7 with pEC-XK99E | This study |
Sorn9 | Sorn7 with pEC-argCJBD1 | This study |
Sorn10 | Sorn7 with pEC-argCJBD2 | This study |
Sorn11 | Sorn7 with pEC-lysE | This study |
Plasmid | ||
pK18mobsacB | Mobilizable vector, allows for selection of double crossover in C. glutamicum, KmR, sacB | [35] |
pEC-XK99E | A shuttle expression vector, KmR | Lab stock |
pK18-ΔargF | A derivative of pK18mobsacB, harboring ΔargF fragment | This study |
pK18-Δncgl1221 | A derivative of pK18mobsacB, harboring Δncgl1221 fragment | This study |
pK18-ΔargR | A derivative of pK18mobsacB, harboring ΔargR fragment | This study |
pK18-ΔputP | A derivative of pK18mobsacB, harboring ΔputP fragment | This study |
pK18-odhA200 | A derivative of pK18mobsacB, harboring odhA of 217 au RBS change and A1G fragment | This study |
pK18-odhA400 | A derivative of pK18mobsacB, harboring odhA of 373 au RBS change and A1G fragment | This study |
pK18-odhA800 | A derivative of pK18mobsacB, harboring odhA of 837 au RBS change and A1G fragment | This study |
pEC-argCJBD1 | A derivative of pEC-XK99E, harboring argCJBD gene from C. glutamicum CICC 20190 under its native promoter | This study |
pEC-argCJBD2 | A derivative of pEC-XK99E, harboring argCJBD gene from C. crenatum MT-M4 under its native promoter | This study |
pEC-lysE | A derivative of pEC-XK99E, harboring lysE gene from C. glutamicum S9114 under its native promoter | This study |