Fig. 2
From: Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae
Pathway construction for de novo biosynthesis of kaempferol in yeast. HPLC analysis of the broth fermented by W303-1A (a), W3NP (b) and W3NP-FF (c) from glucose. W303-1A, wild type; W3NP, a recombinant harboring the pathway of naringenin biosynthesis; W3NP-FF, a recombinant harboring the pathway of kaempferol biosynthesis. Peak 1, dihydrokaempferol; peak 2, kaempferol; peak 3, naringenin. Organization of expressed gene clusters are shown at the top of the chromatogram. P1, ADH1 promoter; P2, HXT7 promoter; P3, PGI1 promoter; P4, TDH3 promoter; T1, TPI1 terminator; T2, TPG1 terminator; T3, ADH1 terminator; T4, FBA1 terminator; T5 PDC1 terminator; T6, RPS2 terminator; T7, TDH1 terminator