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Table 1 Comparison of shikimate production in different recombinant E. coli strains

From: Novel technologies combined with traditional metabolic engineering strategies facilitate the construction of shikimate-producing Escherichia coli

E. coli strains

Engineering strategies

Culture methods

Carbon source

Shikimate production

References

Titer (g/L)

Yield (g/g)

SP1.1pts/pSC6.090B

RB791 (ΔptsHΔptsIΔcrr, serA::aroB, aroL478::Tn10, aroK17::CmR)/pSU18-P tac glfglk-aroF FBR-tktA-P tac aroE-serA

10-L fed-batch fermentation

Glucose

87

0.348

[20]

SP1.1/pKD12.138

RB791 (ΔptsHΔptsIΔcrr, serA::aroB, aroL478::Tn10, aroK17::CmR)/pSU18-aroF FBR-P tac aroE-serA-tktA

2-L fed-batch fermentation

Glucose

52

0.174

[22]

SA116

BW25113 (ΔaroKΔaroLΔrecA, Ppps::PlacQ1, PcsrB::PlacQ1,) containing aroG FBR-tktA-aroB-aroE gene cluster integrated by CIChE and an additional chromosomal copy of nadK

Batch fermentation

Glucose

3.12

0.319

[23]

PB12.SA22

JM101 (ΔptsHIcrrΔaroL ΔaroK, glc+)/pJLB-aroG FBR-tktA and pTOPO -aroB-aroE

1-L batch fermentation

Glucose

7.05

0.29

[6]

SA5/pTH-aroGfbr-ppsA-tktA

B0013 (ΔaroLΔaroK, ΔptsGΔydiBΔackAΔpta)/pTH18kr-aroG FBR-ppsA-tktA

7-L fed-batch fermentation

Glucose

14.6

0.293

[5]

P-9

BW25113 (ΔaraCΔptaΔptsGΔaroLΔtrpRΔpykF) with the wild aroK promoter replaced by the tunable switch/pUC19-aroE-aroD-aroB and pCL1920-aroG FBR-tktA

5-L fed-batch fermentation

Glucose

13.15

0.204

[12]

SA5/pGBAE

BW25113 (ΔaroLΔaroK, ΔptsHIcrr::aroG-aroB- tktA-aroE-glk-galP, ΔtyrR::ppsA)/pETDuet-1 -P T7 -aroG-aroB-P T7 -tktA-aroE

5-L fed-batch fermentation

Glucose and glycerol

27.41

Na

[31]

SK5/pSK6

BW25113 (ΔaroLΔaroKΔydiBΔppcΔldhA)/pSA40-aroB-tktA-aroG FBR-DHQ/SDH-rpsM-aroK

Batch fermentation

Glycerol

5.33

N

[8]

DH5a-T7-P-DK

BL21 (DE3)/pET-28a-glpD-glpK and pAOC-aroE-aroB-glk-tktA-aroF FBR

Batch fermentation

Glycerol

0.2

0.175

[44]

BW25113 (ΔaroLΔaroK, DE3)/pETDuet-GBAE

BW25113 (ΔaroLΔaroK, DE3)/pETDuet-1-aroG-aroB-tktA-aroE

Batch fermentation

Sorbitol

1.0776

0.192

[45]

DHPYAAS-T7

DH5α (ΔptsHIcrr ΔaroLΔydiBΔaroK)/pAOC-aroE-aroB-glk-tktA-aroF FBR

10-L fed-batch fermentation

Glycerol

1.85

0.093

[24]

  1. aNot indicated in the reference