E. coli strains | Engineering strategies | Culture methods | Carbon source | Shikimate production | References | |
---|---|---|---|---|---|---|
Titer (g/L) | Yield (g/g) | |||||
SP1.1pts/pSC6.090B | RB791 (ΔptsHΔptsIΔcrr, serA::aroB, aroL478::Tn10, aroK17::CmR)/pSU18-P tac glfglk-aroF FBR-tktA-P tac aroE-serA | 10-L fed-batch fermentation | Glucose | 87 | 0.348 | [20] |
SP1.1/pKD12.138 | RB791 (ΔptsHΔptsIΔcrr, serA::aroB, aroL478::Tn10, aroK17::CmR)/pSU18-aroF FBR-P tac aroE-serA-tktA | 2-L fed-batch fermentation | Glucose | 52 | 0.174 | [22] |
SA116 | BW25113 (ΔaroKΔaroLΔrecA, Ppps::PlacQ1, PcsrB::PlacQ1,) containing aroG FBR-tktA-aroB-aroE gene cluster integrated by CIChE and an additional chromosomal copy of nadK | Batch fermentation | Glucose | 3.12 | 0.319 | [23] |
PB12.SA22 | JM101 (ΔptsHIcrrΔaroL ΔaroK, glc+)/pJLB-aroG FBR-tktA and pTOPO -aroB-aroE | 1-L batch fermentation | Glucose | 7.05 | 0.29 | [6] |
SA5/pTH-aroGfbr-ppsA-tktA | B0013 (ΔaroLΔaroK, ΔptsGΔydiBΔackAΔpta)/pTH18kr-aroG FBR-ppsA-tktA | 7-L fed-batch fermentation | Glucose | 14.6 | 0.293 | [5] |
P-9 | BW25113 (ΔaraCΔptaΔptsGΔaroLΔtrpRΔpykF) with the wild aroK promoter replaced by the tunable switch/pUC19-aroE-aroD-aroB and pCL1920-aroG FBR-tktA | 5-L fed-batch fermentation | Glucose | 13.15 | 0.204 | [12] |
SA5/pGBAE | BW25113 (ΔaroLΔaroK, ΔptsHIcrr::aroG-aroB- tktA-aroE-glk-galP, ΔtyrR::ppsA)/pETDuet-1 -P T7 -aroG-aroB-P T7 -tktA-aroE | 5-L fed-batch fermentation | Glucose and glycerol | 27.41 | Na | [31] |
SK5/pSK6 | BW25113 (ΔaroLΔaroKΔydiBΔppcΔldhA)/pSA40-aroB-tktA-aroG FBR-DHQ/SDH-rpsM-aroK | Batch fermentation | Glycerol | 5.33 | N | [8] |
DH5a-T7-P-DK | BL21 (DE3)/pET-28a-glpD-glpK and pAOC-aroE-aroB-glk-tktA-aroF FBR | Batch fermentation | Glycerol | 0.2 | 0.175 | [44] |
BW25113 (ΔaroLΔaroK, DE3)/pETDuet-GBAE | BW25113 (ΔaroLΔaroK, DE3)/pETDuet-1-aroG-aroB-tktA-aroE | Batch fermentation | Sorbitol | 1.0776 | 0.192 | [45] |
DHPYAAS-T7 | DH5α (ΔptsHIcrr ΔaroLΔydiBΔaroK)/pAOC-aroE-aroB-glk-tktA-aroF FBR | 10-L fed-batch fermentation | Glycerol | 1.85 | 0.093 | [24] |