Contribution of single amino acid and codon substitutions to the production and secretion of a lipase by Bacillus subtilis

Table 2 Constraint network analysis (CNA) of wtLipA and LipA variants

LipA variants	\(\widetilde{rc}_{{{\text{ij, }}neighbor}}\) (K)^a	\(\Delta \widetilde{rc}_{{{\text{ij, }}neighbor}}\) (K)^b
wtLipA	316.1	–
N50D	312.1	−4.0
P53D	316.2	0.1
P53E	315.8	−0.3
P53 V	315.8	−0.3
R57T	314.9	−1.2
M134Q	314.7	−1.4

^aThe \(\widetilde{rc}_{{{\text{ij, }}neighbor}}\) values were converted to a temperature scale according to equation 4 in Ref. [46]
^bDifference of \(\widetilde{rc}_{{{\text{ij, }}neighbor}}\) values of LipA variants minus wtLipA, respectively

ISSN: 1475-2859