Bacterial strains and plasmids | Genotype | References |
---|---|---|
Bacterial strains | ||
E. coli DH5α | supE44 ∆(lacZYA-argF) U196 (phi80∆lacZM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | [20] |
B. subtilis TEB1030 | trpC2 his nprE aprE bpf ispI lipA lipB | [19] |
Plasmids | ||
pBSMul1 | E. coli–B. subtilis shuttle vector, ribosome binding site, P HpaII , secretion (sslipA) and purification (C-terminal 6x-His-tag); ColE1 repB Km r Amp r | [21] |
pET22lipA | pET22b (Novagen, USA) containing a 557Â bp EcoRV/SacI fragment of B. subtilis lipA gene fused to pelB signal peptide sequence, PT7lac | [18] |
pBSlipA | pBSMul1 containing a 568Â bp EcoRI/HindIII fragment of B. subtilis lipA gene; additionally deleted EcoRI restriction site | This study |