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Table 1 Bacterial strains and plasmids

From: Contribution of single amino acid and codon substitutions to the production and secretion of a lipase by Bacillus subtilis

Bacterial strains and plasmids

Genotype

References

Bacterial strains

E. coli DH5α

supE44 ∆(lacZYA-argF) U196 (phi80∆lacZM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

[20]

B. subtilis TEB1030

trpC2 his nprE aprE bpf ispI lipA lipB

[19]

Plasmids

pBSMul1

E. coli–B. subtilis shuttle vector, ribosome binding site, P HpaII , secretion (sslipA) and purification (C-terminal 6x-His-tag); ColE1 repB Km r Amp r

[21]

pET22lipA

pET22b (Novagen, USA) containing a 557 bp EcoRV/SacI fragment of B. subtilis lipA gene fused to pelB signal peptide sequence, PT7lac

[18]

pBSlipA

pBSMul1 containing a 568 bp EcoRI/HindIII fragment of B. subtilis lipA gene; additionally deleted EcoRI restriction site

This study