Pseudomonas stutzeri as an alternative host for membrane proteins

Table 2 Electroporation of P. stutzeri

Donor strain	Culture medium for recipient strain (P. stutzeri ZoBell)	Treatment	Transformants per μg plasmid DNA
P. stutzeri ZoBell	LB medium	/	10⁶–10⁷
E. coli DH5a or JM110	LB medium	/	10³–10⁴
E. coli DH5a	Asn medium	/	10²–10³
E. coli DH5a	LB medium	In vitro methylation using P. stutzeri cell-free extracts^a	10³–10⁴
E. coli DH5a	LB medium	Electroporated with TypeOne restriction inhibitor^b	10³–10⁴

Electroporation was performed with 100 ng of plasmid DNA as described in “Electroporation of Pseudomonas stutzeri ”
^aPlasmid was treated with P. stutzeri cell-free extracts prepared as described [28, 29]
^bTypeOne restriction inhibitor was purchased from Epicentre

ISSN: 1475-2859