Fig. 5
From: Pseudomonas stutzeri as an alternative host for membrane proteins
Workflow to screen membrane protein production. The screening procedure is based on a single vector capable to drive expression in several Gram-negative hosts. Due to the serial cloning strategy the number of generated constructs is reduced to a minimum. The workflow was applied with P. stutzeri as the production host and yielded 20 out of 36 proteins to be produced at high levels