Fig. 3

GFP folding assay in E. coli TOP10 and P. stutzeri ZoBell. a Production of seven GFP-fused membrane proteins was induced with four different l-arabinose concentrations (w/v). 35 µg of total protein were analyzed on SDS-PAGE, followed by in-gel fluorescence detection and Western blotting performed against the His tag. The “+” and “−” signs indicate the positions of fluorescent and nonfluorescent species of the proteins, respectively. b Direct quantitative comparison of protein folding in E. coli (Ec) and P. stutzeri (Ps). Protein production was induced with 0.02% (w/v) l-arabinose