Fig. 2
From: Pseudomonas stutzeri as an alternative host for membrane proteins
pL2020 is a broad host range vector enabling regulated and dose-dependent protein production in Gram-negative bacteria. It is based on the pBBR1MCS1 plasmid and utilizes the araC/PBAD system. a Plasmid map of pL2020. araC, regulater protein; PBAD, l-arabinose inducible promoter; MCS, multiple cloning site; rrnB, transcription termination sequence; CamR, chloramphenicol resistance gene; mob, mobility element; pBBR, broad host range origin of replication. b Multiple cloning site of the pL2020 vector. The inverse architecture facilitates cloning of N- or C-terminally tagged His10 fusions. Upon purification the affinity tag can be removed by TEV cleavage