Table 3 Primers used in this study
Primers | Sequence (5′–3′) | Restriction sites |
|---|---|---|
HisF | AAAGAATTCTAAAGTAATTTTCATCAATTTTTTCTAAGC | EcoRI |
HisR | GTTTGGGAGTCGCCTTTGGCTC | – |
TsF | AAAGGATCCTGATCGTTAAATTTATACTGCAAT | BamHI |
TsR | AAACTGCAGTACCTAATAATTTATCTACATTCCC | PstI |
PZF | AAAGAATTCAGTCTTATAACTATACTGACAATAG | EcoRI |
PZR | TGATAATTGTGTCATTTTGAGTGCCTCCTTATAAT | – |
LF | AAGGAGGCACTCAAAATGACACAATTATCACGTTT | – |
LR | AAATCTAGACTAATTTCTCAATACTTGAACAT | XbaI |
P32Rec | AAGTCGCGTGGCGCGCCGGTCCTCGGGATATGATA | AscI |
PZRec | AAGTCGCGTGGCGCGCCAGTCTTATAACTATACTGACAATAG | AscI |
TerRec | GAAATGATAGGCGCGCCATAAGCAAAAGGCAGCTGAT | AscI |
LO | GCTCCATGAATTTCCTAATTGATGC | – |
RO | GATGAAGCTTATATTGACTTTGGCG | – |