Fig. 4

Confirmation analysis of the five HDFs knocked-in strains constructed through the knock-in reporter system. a Schematic of genomes of the NZ9000, NZB, and the five HDF knocked-in strains. LO, RO, LF, LR, P32Rec, PZRec, and TerRec as primers used in Multiple-PCR analysis. b Confirmation analysis of the HDFs knocked-in strains by multiple-PCR analysis. The primer combinations used in PCR are presented on the lanes. Genomic DNA from the following strains were used as templates: 1.3k-NZ (lanes 1, 8, and 15), 2.2k-NZ (lanes 2, 9, and 16), 3.8k-NZ (lanes 3, 10, and 17), 7.3k-NZ (lanes 4, 11, and 18), 14.6k-NZ (lanes 5, 12, and 19), NZB (lanes 6, 13, and 20) and NZ9000 (lanes 7, 14, and 21). The 1 kb DNA ladder marker is shown on the left (M1), and the λ/HindIII marker is shown on the right (M2). The theoretical size (bp) of each PCR products generated with the primer combinations is shown in Additional file 1: Table S1. HDF, heterologous DNA fragment; LO, RO, LF, LR, P32Rec, PZRec, and TerRec as the primers used in Multiple-PCR analysis