Fig. 2

Confirmation analysis of the NZB strain. a Schematic of genomes of NZ9000 and NZB. LO, RO, LF, LR, primers used in multiple-PCR analysis. b Multiple-PCR analysis of the NZB and NZ9000 strain. The primer combinations used in PCR are presented on the lanes. Genomic DNA from the following strains were used as templates: NZB (lanes 1 and 3) and NZ9000 (lanes 2 and 4). The 1 kb DNA ladder marker is shown on the left (M). The theoretical size (bp) of each PCR products generated with the primer combinations is shown in Additional file 1: Table S1. c NZ9000 (left) and NZB (right) colonies on M17GS-XN