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Table 5 Strain specific, physiological parameters determined in FB3

From: A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris

Phase

qs,gly (g g−1 h−1)

qs,MeOH (g g−1 h−1)

µ (h−1)

qp (mg g−1 h−1)

\({\text{Y}}_{{{{{\text{CO}}_{2} } \mathord{\left/ {\vphantom {{{\text{CO}}_{2} } {\text{S}}}} \right. \kern-0pt} {\text{S}}}}}\) (Cmol Cmol−1)

YX/S (Cmol Cmol−1)

C-balance (−)

Glycerol

0.212

–

0.122

0.368

0.40

0.57

0.97

Methanol

–

0.034

0.003

0.097

0.83

0.15

0.98

Mixed feed

0.135

0.035

0.106

0.573

0.54

0.41

0.95