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Table 4 Dynamics in FB2 to analyze tunability and to find an operating window for the bi-directional promoter system allowing highest qp

From: A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris

Steps

qs,gly (g g−1 h−1)

qs,MeOH (g g−1 h−1)

µ (h−1)

qp (mg g−1 h−1)

\({\text{Y}}_{{{{{\text{CO}}_{2} } \mathord{\left/ {\vphantom {{{\text{CO}}_{2} } {\text{S}}}} \right. \kern-0pt} {\text{S}}}}}\) (Cmol Cmol−1)

YX/S (Cmol Cmol−1)

C-balance (−)

1

0.025

0.022

0.005

0.109

0.78

0.14

0.92

2

0.059

0.024

0.009

0.372

0.71

0.23

0.94

3

0.139

0.025

0.031

0.349

0.55

0.42

0.97

4

0.239

0.023

0.112

0.099

0.42

0.57

0.99