Fig. 2From: A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris SDS-PAGE gel of cell-free cultivation broth taken at different time points during FB3. Lane 1, protein ladder; lane 2, sample after batch on glycerol; lanes 3–5, samples during fed-batch at qs,gly = 0.212 g g−1 h−1 (samples were taken every 2 h); lane 6, sample after methanol fed-batch; lane 7, sample after mixed feed phase. Size CBH2 = ca. 60 kDaBack to article page