Fig. 7
Effect of temperature on the activity and stability of the soluble and immobilized Est684. a The optimum temperature of the soluble (filled square) and immobilized (open square) Est684 was determined analogously by measuring esterase activity in the temperature range of 0–50 °C in a phosphate buffer (50 mM, pH 6.5). b Thermostability of the soluble (red line) and immobilized (black line) Est684 was measured by preincubation of enzymes in 50 mM potassium phosphate buffer (pH 6.5) at 18 (filled square) and 35 °C (filled triangle) for different intervals. Data points are the average of triplicate measurements, and error bars represent the standard deviation