Fig. 3
Effect of temperature on the activity and stability of Est684. a The optimum temperature was determined analogously by measuring esterase activity in the temperature range of 0–50 °C in a phosphate buffer (50 mM, pH 6.5). b Thermostability was measured by preincubation of enzymes in 50 mM potassium phosphate buffer (pH 6.5) at 18 (filled square), 25 (filled circle), 30 (filled triangle) and 35 °C (open triangle) for different intervals. Data points are the average of triplicate measurements, and error bars represent the standard deviation