Fig. 1

a Western blotting analysis of spore adsorption with purified FliD protein. Upon adsorption with purified FliD spore surface proteins were extracted by SDS-DTT treatment, fractionated on SDS-PAGE and analyzed by Western blotting. Spore coat extracts were prepared with spores of wild-type strain 168 and recombinant BKH121 alone or with adsorption with FliD (indicated with − and +). Purified FliD (1 μg) was used as positive control. b The same volumes of spore coat extracts were simultaneously loaded onto the second SDS-PAGE gel and analyzed by Western blotting using anti-CotZ antibodies to verify the equality of protein loading. The calculated molecular mass of CotZ is 16.4 kDa