Fig. 2

Effect of additional GLD expression on product formation over 24 h, using whole cells, mechanically broken cells or cell free extract. Cultures were at a high cell density, and produced octyl acetate by-product alongside 1-octanol (an artefact of the expression system). The product concentrations shown here are the combined concentrations of 1-octanol and octyl acetate. Two vials were sacrificed to obtain each sample point. The organic phase was extracted into ethyl acetate and analysed via gas chromatography