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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica

Fig. 8

Effect of carotenoid and lipid synthesis inhibitors on recombinant protein expression, under control of SSIP1 promoter, in T. pseudonana after 24 h of Si starvation. Expression of eGFP was measured using an imaging flow cytometer (488 nM laser/100 mW with neutral density filter 0.6). Numbers under bars indicate concentrations of the inhibitors dithiothreitol (DTT): mM, isoxaflutole (IFT): µg/mL, norflurazon (NF): µM, sethoxydim (Sethox): µM. a eGFP intensity/cell; b Net eGFP intensity; c percentage of cells expressing eGFP. Error bars represent standard errors of mean (SEM), n = 20,000. Control sample was without any addition of inhibitors

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