Fig. 8From: Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica Effect of carotenoid and lipid synthesis inhibitors on recombinant protein expression, under control of SSIP1 promoter, in T. pseudonana after 24 h of Si starvation. Expression of eGFP was measured using an imaging flow cytometer (488 nM laser/100 mW with neutral density filter 0.6). Numbers under bars indicate concentrations of the inhibitors dithiothreitol (DTT): mM, isoxaflutole (IFT): µg/mL, norflurazon (NF): µM, sethoxydim (Sethox): µM. a eGFP intensity/cell; b Net eGFP intensity; c percentage of cells expressing eGFP. Error bars represent standard errors of mean (SEM), n = 20,000. Control sample was without any addition of inhibitorsBack to article page