Fig. 4

Comparison of eGFP expression driven by Si inducible and non Si inducible promoters in Si-replete media and after 24 h of Si starvation. The net eGFP fluorescence was calculated as product of eGFP intensity/cell and no. of cells expressing eGFP derived from imaging flow cytometry. To compensate for different levels of expression, eGFP was measured with a 488-nm laser with 100 mW power output without filter (TpNRp, TprpL41p, TpFcpp) and 30 mW power output after blocking saturation with neutral density filter 1.0 (TpSIT1p, TPSIT2p, Thaps3_9619p, and CcSITp). Error bars represent standard errors of mean (SEM), n = 20,000. NR nitrate reductase promoter, rpL41p ribosomal protein L41 promoter, Fcpp fucoxanthin chlorophyll a/c binding protein promoter