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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica

Fig. 3

Induction level of T. pseudonana and C. cryptica SSIPs in Si-replete media and after 24 h of Si starvation as determined by imaging flow cytometry. a Mean eGFP fluorescence per cell. b Net eGFP fluorescence calculated as product of eGFP intensity/cell and no. of cells expressing eGFP. c Percentage of cells expressing eGFP. eGFP was measured with a 488-nm laser with 50 mw power output without a blocking filter. Error bars represent standard errors of mean (SEM), n = 20,000. TpSIT1p, Thaps3_268895; TpSIT2p, Thaps3_41392; Tp9619p, Thaps3_9619; and CcSIT1p, g10780.t1

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Microbial Cell Factories

ISSN: 1475-2859

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