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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus

Fig. 5

Comparison of the cleavage efficiency of gene cluster guided by four spacers under different conditions. a Four spacer positions (11-1 to 11-4) flanking the myxovirescin gene cluster were used as the sgRNA-targeting sites for deletion. The genes involved in the biosynthesis of myxovirescin are shown in the arrows. The positions of the guide sequences are indicated. b Histograms show the target cleavage efficiencies of the four sgRNAs including the 11-1 to 11-4 spacers, respectively, controlled with different promoters or different concentrations of copper ion. Experiments were performed in triplicate. Error bars indicate standard deviations

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