Table 1 List of strains and plasmids used in this study
From: New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica
Strain or (plasmid) | Genotype or other relevant characteristics | Source or reference |
|---|---|---|
E. coli | ||
DH5α | ϕ80dlacZ∆m15, recA1, endA1, gyrA96, thi-1, hsdR17 (r k −, m k +), supE44, relA1, deoR, Δ(lacZYA-argF)U169 | Promega |
pCR4Blunt-TOPO® | Cloning vector, kanamycin | Invitrogen |
pJET 1.2 | Cloning vector, ampiciline | Thermo scientific |
JME507 | JMP113 (1.2 kb ylLEU2 fragment, LEU2ex marker) | [41] |
JME461 | pRRQ2 (Cre ARS68 LEU2) | [53] |
JME507 | JMP113, LEU2ex | [41] |
JME547 | pUB4-CRE | [41] |
JME803 | JMP62-pPOX2-URA3ex | [54] |
JME1427 | JMP62-pTEF-YFP-LEU2ex | B Treton, unpublished, BIMLip/INRA |
JMP62-php4d-YFP-URA3ex | B Treton, unpublished BIMLip/INRA | |
JME2027 | pCR4Blunt-TOPO—ClaI-4UAS1xpr2-BstBI | [33] |
JME4123 | PUC57-pEYK300A3B | GenScript, Hong-Kong |
JME4124 | PUC57-pEYK300A3b | GenScript, Hong-Kong |
FCP007 | pJET 1.2-pEYK300; ClaI-BamHI | This work |
JME3934 (FCP013) | JMP62-pEYK300-YFP-URA3ex | This work |
JME3994* (JMP3994)** | JMP62-pEYK450-YFP-URA3ex | This work |
JME3988* (JMP3988)** | JMP62-pEYK300aB-YFP-URA3ex | This work |
JME3991* (JMP3991)** | JMP62-pEYK300Ba-YFP-URA3ex | This work |
JME3998* (JMP3998)** | JMP62-pHU4-EYK300-YFP-URA3ex | This work |
JME4137* (JMP4137)** | JMP62-pEYK300A3B-YFP-URA3ex | This work |
JME4139* (JMP4139)** | JMP62-pEYK300A3b-YFP-URA3ex | This work |
Y. lipolytica | ||
W29 | MATA, wild-type | [35] |
Po1d | MATA ura3-302 leu2-270 xpr2-322 | [35] |
JMY330 | Po1d, Ura+ | [54] |
JMY2101 | Po1d, Leu+ | [33] |
JMY2900 | Po1d, Ura+ Leu+ | [33] |
RIY147 | Po1d eyk1::LEU2ex, Ura− | This work |
RIY176 | Po1d eyk1∆ Ura− Leu− | This work |
JMY2876 | JMY330 + pTEF-YFP-LEU2ex (Ura+ Leu+) | B. Treton unpublished BIMLip/INRA |
JMY6245 (FCY003) | JMY2101 + pEYK300-YFP-URA3ex (Ura+ Leu+) | This work |
JMY6369 | JMY2101 + pEYK300aB-YFP-URA3ex (Ura+ Leu+) | This work |
JMY6372 | JMY2101 + pEYK300Ab-YFP-URA3ex (Ura+ Leu+) | This work |
JMY6375 | JMY2101 + pEYK450-YFP-URA3ex (Ura+ Leu+) | This work |
JMY6380 | JMY2101 + pHU4-EYK300-YFP-URA3ex (Ura+ Leu+) | This work |
JMY6681 | JMY2101 + pEYK300A3B-YFP-URA3ex (Ura+ Leu+) | This work |
JMY6684 | JMY2101 + pEYK300A3b-YFP-URA3ex (Ura+ Leu+) | This work |
RIY180 (JMY6637) | RIY176 + pEYK300-YFP-LEU2ex (Ura− Leu+) | This work |