Fig. 3
From: New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica
Schematic representation of plasmids constructed in this study. Plasmid pEYK300 contained the yellow fluorescent protein YFP, under the 285 bp promoter region of the EYK1 gene (erythrulose kinase; YALI0F01606g). The vectors contain the zeta sequence for targeting the expression cassette obtained after NotI digestion. KanR and URA3 markers are for selection in E. coli and Y. lipolytica, respectively. The URA3 is flanked by LoxP/LoxR region for marker rescue (excisable marker URA3ex). JME3934 (pEYK300-YPF); JME3994 (pEYK450-YFP); JMP3988 (pEYK300aB-YPF); JMP3991 (pEYK300Ab-YPF); JMP4137 (pEYK300A3B-YPF)-YPF; JMP4139 (pEYK300A3b-YPF) and JMP3998 (pHU4EYK300-YPF)