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Fig. 2 | Microbial Cell Factories

Fig. 2

From: New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica

Fig. 2

Schematic representation of promoters used in this study. Schematic representation of the genomic locus containing the upstream gene YALI0F01628g and the EYK1 gene, YALI0F01606g. a Schematic representation of the native promoters pEYK450 (TATA box + native A box + native B box) and pEYK300 (native A box + native B box) controlling the expression of YFP; b Schematic representation of the mutated promoters pEYK300aB (mutated A box + native B box) and pEYK300Ab (native A box + mutated B box) controlling the expression of YFP; c Schematic representation of the hybrid promoters pEYK300A3B (3 A boxes + native B box) and pHu4EYK300 (4 tandem copies of UAS1xpr2 + native A box + native B box) controlling the expression of YFP. Symbols are white filled square: TATA box (T), black filled square: A box (A), grey filled square: B box (B), black filled triangle: mutated A box, grey filled triangle: mutated B box, grey rightwards arrow: YFP gene, rectangle: four tandem copies of UAS1xpr2

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