Fig. 2

A flow chart of rhamnolipids production. This flow chart shows the procedures for rhamnolipid production. A large scale rhamnolipid production contains the following steps: seed preparation, fermentation, production purification. For a given strain, the costs are focused on the fermentation and purification steps. Seed preparation for fermentation is a common step which does not require extra cost. Fermentation cost includes carbon and nitrogen sources, fermentation styles, and other additional steps or chemicals required for rhamnolipid production. Cost for production purification depends on the required production purity. Some applications may not require rhamnolipid purification