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Table 2 Obtained results for the reductive amination of KMV with and without addition of amine donor l-alanine

From: In vivo plug-and-play: a modular multi-enzyme single-cell catalyst for the asymmetric amination of ketoacids and ketones

Catalyst

Conversion [%] without l-alanine

Conversion [%] with l-alanine

Cofactor regeneration

Enzyme-coupled

Metabolic

Enzyme-coupled

Metabolic

3a (Ec-AlaDH/pTrc99a-avtA/pBADΔbla-fdh)

8

8

88a

94a

3b (Ec-AlaDH/pTrc99a-ald-avtA/pBADΔbla-fdh)

13

12

88b

96b

4a (Ec-AlaDH/pTrc99a-avtA/pBADΔbla-gdh)

44

54

73a

78a

4b (Ec-AlaDH/pTrc99a-ald-avtA/pBADΔbla-gdh)

80

84

90b

94b

5a (Ec-AlaDH/pTrc99a-avtA/pBADΔbla-ptdh)

7

7

90a

87a

5b (Ec-AlaDH/pTrc99a-ald-avtA/pBADΔbla-ptdh)

5

6

54c

58c

  1. Conversion obtained for the enzyme-coupled system for cofactor regeneration was compared with the metabolic one
  2. aAddition of 250 mM l-alanine
  3. bAddition of 50 mM l-alanine
  4. cAddition of 100 mM l-alanine