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Fig. 6 | Microbial Cell Factories

Fig. 6

From: In vivo plug-and-play: a modular multi-enzyme single-cell catalyst for the asymmetric amination of ketoacids and ketones

Fig. 6

Activities of singe-cell catalysts for “in vitro-type” amination of ketones. Initial rates of single-cell catalysts 6 (Ec-AlaDH/pTrc99A-ta Cv /pBAD28Δbla-fdh), 7 (Ec-AlaDH/pTrc99A-ta Cv /pBAD28Δbla-gdh) and 8 (Ec-AlaDH/pTrc99A-ta Cv /pBAD28Δbla-ptdh) in the reductive amination of 4-phenyl-2-butanone (25 mM), coupling TaCv with AlaDH and either FDH, GDH or PtDH for cofactor regeneration. Typical time curves are shown for the production of 4-phenyl-2-butylamine over a time period of 60 or 120 min, respectively using 50 mg resting cells per mL

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Microbial Cell Factories

ISSN: 1475-2859

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