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Fig. 4 | Microbial Cell Factories

Fig. 4

From: In vivo plug-and-play: a modular multi-enzyme single-cell catalyst for the asymmetric amination of ketoacids and ketones

Fig. 4

Fermentative amination of α-ketoacids with metabolic NADH regeneration. Fermentation of KIC to l-leucine a with catalyst 1a, 1b (Ec-GluDH carrying pTrc99A-ilvE Ec or pTrc99A-ilvE Sm ) and KMN to l-isoleucine b with catalyst 2a, 2b, 2c (Ec-AlaDH carrying pTrc99A-avtA, pTrc99A-avta-ald or pTrc99A-ald-avtA). SGA medium was inoculated aerobically with recombinant strains for the reductive amination of 50 mM KIC or KMV to l-leucine and l-isoleucine, respectively. 100 mM glucose, 50 mM (NH4)2SO4 and 0 or 250 mM l-glutamate in case of catalyst 1a–b were added to shift the reaction equilibrium towards product side

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