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TableĀ 4 Comparison of normalized data from five different analytical methods to evaluate cell disruption efficiency

From: A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization

Cycle Total protein content [%] Signal reduction [%] Viable cells [%]
Bradford HPLC DS CfUs FC
0 10.2 15.0 100.0 100.0 100.0
1 83.8 88.8 13.9 18.3 21.9
2 93.7 96.9 8.7 13.8 15.1
3 99.2 99.3 8.3 13.1 14.1
4 100.3 99.5 7.4 11.0 13.6
5 100.0 100.0 7.1 10.7 11.1
  1. The total protein content after five cycles was considered to be 100%. Based on this assumption %-values for the other cycles were calculated