Skip to main content

Table 4 Comparison of normalized data from five different analytical methods to evaluate cell disruption efficiency

From: A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization

Cycle

Total protein content [%]

Signal reduction [%]

Viable cells [%]

Bradford

HPLC

DS

CfUs

FC

0

10.2

15.0

100.0

100.0

100.0

1

83.8

88.8

13.9

18.3

21.9

2

93.7

96.9

8.7

13.8

15.1

3

99.2

99.3

8.3

13.1

14.1

4

100.3

99.5

7.4

11.0

13.6

5

100.0

100.0

7.1

10.7

11.1

  1. The total protein content after five cycles was considered to be 100%. Based on this assumption %-values for the other cycles were calculated