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Table 3 Overview of the three experimental work packages (WPs)

From: A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization

WP Strategy Analytics Goal
1 DCW: 10 g/L
Pressure: 1500 bar
Cycles: 0–5
Bradford = state-of-the-art
Flow cytometry = state-of-the-art
CfUs = state-of-the-art
Dielectric spectroscopy = additional method
HPLC and automated data analysis = novel method
Evaluation of applicability and accuracy of our method
2 DCW: 10 g/L
Pressure: 1500 bar
Cycles: 0–5
Bradford
HPLC and automated data analysis
Analyzing potential effects of freezing on cell disruption efficiency
3 DCW: 10–100 g/L
Pressure: 500–1500 bar
Cycles: 0–3
Bradford
HPLC and automated data analysis
Evaluation the effect of BM concentration, pressure and cycles on cell disruption