Table 3 Overview of the three experimental work packages (WPs)
WP | Strategy | Analytics | Goal |
|---|---|---|---|
1 | DCW: 10 g/L Pressure: 1500 bar Cycles: 0–5 | Bradford = state-of-the-art Flow cytometry = state-of-the-art CfUs = state-of-the-art Dielectric spectroscopy = additional method HPLC and automated data analysis = novel method | Evaluation of applicability and accuracy of our method |
2 | DCW: 10 g/L Pressure: 1500 bar Cycles: 0–5 | Bradford HPLC and automated data analysis | Analyzing potential effects of freezing on cell disruption efficiency |
3 | DCW: 10–100 g/L Pressure: 500–1500 bar Cycles: 0–3 | Bradford HPLC and automated data analysis | Evaluation the effect of BM concentration, pressure and cycles on cell disruption |