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Table 2 Common methods to evaluate cell disruption efficiency

From: A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization

Answer Method Advantage Disadvantage References
Total protein release Protein concentration (e.g. Bradford, Lowry) Relatively fast, standard lab equipment sufficient Matrix interference, manual intervention [8, 15, 18, 21]
Cell viability Microscope/flow cytometry Detailed information Error prone, dyes needed, expensive [20, 21]
Plate out (Colony forming Units (CfUs)) Standard lab equipment sufficient Error prone, time consuming, laborious [18, 20, 22, 23]
Product specific assays SDS-Page, Western blot, ELISA, enzyme assays Product specific Time consuming, laborious, manual intervention [12, 13, 15, 24]
Particle size distribution Light scattering (e.g. Coulter Multisizer II, Nanophox PCCS) Detailed information Manual intervention, time consuming [13, 18, 19, 24]