A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization

Table 2 Common methods to evaluate cell disruption efficiency

Answer	Method	Advantage	Disadvantage	References
Total protein release	Protein concentration (e.g. Bradford, Lowry)	Relatively fast, standard lab equipment sufficient	Matrix interference, manual intervention	[8, 15, 18, 21]
Cell viability	Microscope/flow cytometry	Detailed information	Error prone, dyes needed, expensive	[20, 21]
Cell viability	Plate out (Colony forming Units (CfUs))	Standard lab equipment sufficient	Error prone, time consuming, laborious	[18, 20, 22, 23]
Product specific assays	SDS-Page, Western blot, ELISA, enzyme assays	Product specific	Time consuming, laborious, manual intervention	[12, 13, 15, 24]
Particle size distribution	Light scattering (e.g. Coulter Multisizer II, Nanophox PCCS)	Detailed information	Manual intervention, time consuming	[13, 18, 19, 24]

ISSN: 1475-2859