Table 1 Selected gRNAs and their target replicons
From: A versatile one-step CRISPR-Cas9 based approach to plasmid-curing
gRNA | Sequence (5′ to 3′) | Targeted replicon group |
|---|---|---|
gRNA1 | ATGAACTAGCGATTA GTCGCTATGACTTAA | pSC101 |
gRNA2 | AACCACACTAGAGAA CATACTGGCTAAATA | pSC101 |
gRNA3 | GGTTGGACTCAAGAC GATAGTTACCGGATA | ColE1-like except colA |
gRNA4 | GGCGAAACCCGACAG GACTATAAAGATACC | ColE1-like including colA (self-curing of pFREE) |