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Table 1 Kinetic parameters of growth and production of rPLA2 (from Micrurus laticollaris) and of mixed-acid fermentation metabolites by E. coli BL21-Gold (DE3)

From: Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

Parameter

OM (rpm)

RAM (g)

200

7.5

12.5

20

kLa, h−1

78.9 ± 2.0

82.0 ± 10.4

160.4 ± 27.9

287.4 ± 71.6

µ, h−1

0.47 ± 0.06

0.70 ± 0.08

0.73 ± 0.06

0.78 ± 0.06

Xmax, g/L

1.81 ± 0.59

5.82 ± 1.76

9.57 ± 2.26

8.22 ± 2.11

YX/S, g/g

0.22 ± 0.03

0.42 ± 0.05

0.57 ± 0.06

0.48 ± 0.05

qs, g/(g h)

0.40 ± 0.04

0.15 ± 0.04

0.16 ± 0.03

0.14 ± 0.04

t (h), DOT → 0%

10.0 ± 0.5

3.0 ± 0.2

2.0 ± 0.2

2.0 ± 0.2

Ytotal protein/X, g/g

0.43 ± 0.04

0.31 ± 0.02

0.23 ± 0.03

0.24 ± 0.01

% rPLA in insoluble protein

38 ± 5

39 ± 3

37 ± 4

36 ± 5

rPLA2, mg/La

117 ± 15

295 ± 22

240 ± 19

262 ± 22

YrPLA2/X, mg/g

65 ± 7

52 ± 8

27 ± 6

32 ± 6

qacetate, mg/(g h)

60.6 ± 5.1

24.5 ± 3.6

9.7 ± 2.4

3.7 ± 1.2b

qformate, mg/(g h)

0.07 ± 0.02

0.09 ± 0.04

0.25 ± 0.07

0.40 ± 0.08

qsuccinate, mg/(g h)c

22.6 ± 2.1

6.6 ± 1.2

2.8 ± 0.8

4.1 ± 1.1

qmalate, mg/(g h)

2.8 ± 0.7

5.5 ± 0.7

3.9 ± 0.6

3.9 ± 0.5

  1. Mean and standard deviation for at least three independent experiments are presented
  2. aCalculated based on gel densitometry (Fig. 1e) and the insoluble protein obtained
  3. bCalculated before 8 h of culture when acetate was accumulated
  4. cCalculated before 10 h of culture when succinate was accumulated
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Microbial Cell Factories

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