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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

Fig. 5

TEM micrographs of cross-sections of E. coli BL21-Gold (DE3)–rPLA2. a Cells agitated at 200-rpm OM, without induction. b–d Cells agitated at 20g. Samples were collected 13 h post-induction. A black arrowhead shows vesicle-like bodies. The open arrowhead shows budding membrane structures (scale bars of 0.5 µm). e, f TEM micrographs of cross-sections of vesicles concentrated by filtration and centrifugation from different cultures of E. coli BL21-Gold (DE3)–rPLA2 at 20g RAM

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Microbial Cell Factories

ISSN: 1475-2859

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