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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

Fig. 4

a Kinetics comparison of proteinase-K digestion of rPLA2 (from Micrurus laticollaris) IBs purified by E. coli BL21-Gold (DE3), at 200-rpm OM (filled circles) or 7.5g (open triangles), 12.5g (open circles), and 20g RAM (open squares). The degradation was observed by absorbance (350 nm) and data were normalized. Data show the average and standard deviation of IBs purified from three independent cultures for each condition. b Second derivative FTIR spectra of rPLA2 inclusion bodies in amide I band region from cultures carried out at 200-rpm OM (continuous line), 7.5g RAM (dashed line), 12.5g RAM (dotted line), and 20g RAM (dashed-dotted line). Data show the average of triplicate experiments

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Microbial Cell Factories

ISSN: 1475-2859

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