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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

Fig. 1

Kinetics of growth and production of rPLA2 (from Micrurus laticollaris) by E. coli BL21-Gold (DE3), at 200-rpm OM (filled circles) or 7.5g (open triangles), 12.5g (open circles), and 20g RAM (open squares). Data show the average and standard deviation of the cultures carried out at least in triplicate. The dashed line shows the start of IPTG induction (4 h of culture). a Biomass growth. b Glucose consumption. c Dissolved oxygen tension (DOT). d Total protein concentration. e Analysis of insoluble and cytoplasmic soluble proteins by SDS-PAGE (solubilized with 3% SDS) of samples at 13 h post-induction (17 h of culture). f A Western blot of insoluble proteins (solubilized with 3% SDS). Lanes 1, 2, 3, and 4 correspond to insoluble proteins from cultures at 200 rpm OM or RAM at 7.5, 12.5, or 20g, respectively. Lanes 1S, 2S, 3S, and 4S, correspond to cytoplasmic soluble proteins from the same cultures. M means molecular weight markers, and (–) denotes an empty line

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