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Table 1 16S rRNA gene copy numbers and relative abundance of phylotypes VLD-1 and VLD-2

From: Identification of two organohalide-respiring Dehalococcoidia associated to different dechlorination activities in PCB-impacted marine sediments

  Time (weeks) Organic chlorine (µmol/gdw) Expected increasea VLD-1 VLD-2 VLD-1 VLD-2
(16S rRNA gene copies per gram of sediment) (16S rRNA gene copies per gram of sediment) Relative abundance (%)
C 0 23.2 ± 0.4 2.1E+6 (±6.7E+5) 1.5E+7 (±9.8E+6) 0.1 ± 0.0 0.4 ± 0.3
27 20.4 ± 1.5 1.4E+8 (±9.2E+7) 3.5E+8 (±2.7E+8) 7.4E+9 (±1.2E+9) 2.5 ± 1.9 55.9 ± 5.2
D 0 23.2 ± 0.7 9.2E±7 (±9.5E+7) 1.2E+8 (±7.5E+6) 0.6 ± 0.6 0.8 ± 0.1
27 18.6 ± 0.3 2.3E+8 (±5.2E+7) 2.0E+9 (±7.1E+8) 3.1E+8 (±2.1E+8) 15.2 ± 1.0 2.1 ± 0.9
E1 0 23.3 ± 0.1 6.7E+6 (±1.7E+6) 1.3E+7 (±6.3E+6) 1.2 ± 0.4 2.4 ± 1.3
27 19.4 ± 0.2 1.9E+8 (±1.7E+7) 4.8E+8 (±2.2E+7) 1.9E+5 (±4.6E+4) 55.5 ± 2.6 0.0 ± 0.0
E2 0 23.3 ± 0.9 2.6E+6 (±4.2E+6) 5.3E+6 (±3.3E+6) 0.3 ± 0.1 0.5 ± 0.1
27 21.0 ± 0.9 1.1E+8 (±9.2E+7) 3.1E+8 (±3.2E+7) 1.1E+8 (±1.9E+6) 58.2 ± 4.8 22.0 ± 1.7
  1. Organic chlorine, expected increase of 16S rRNA gene copies of PCB respirers, observed 16S rRNA genes copy numbers per gram of sediment and relative abundance (vs total number of bacterial 16S rRNA genes) of phylotypes VLD-1 and VLD-2 at the beginning and after 27 weeks of incubation (27 week). Values are the mean (±SD) of triplicate analysis performed on metagenomic DNA extracted from each replicate culture with primer pairs targeting 16S rRNA genes of individual phylotypes (VLD-1, VLD-2) and total bacteria
  2. aThe expected increase of 16S rRNA gene copy numbers of PCB respirers was calculated from the organic chlorine removed over incubation using the growth yield of D. mccartyi on PCBs reported in Matturro et al. [40]