Fig. 5

SDS-Page and western blot analysis of intracellular versus extracellular secretion of the chimeric TeTrCBH I protein in L. starkeyi. The strain of LS8-7 with signal peptide from L. starkeyi dextranase 2 gene was cultured for this analysis. Lanes 1–3 indicate cell extracts or exoproteomes that were diluted to 9, 3 and 1×, respectively. For cell extracts, the protein was extracted from cell pellets of culture and diluted in series as indicated above. For exoproteomes, culture supernatant was concentrated 35× and diluted in series