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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi

Fig. 3

SDS-PAGE and western blot analysis of the overexpression of chimeric TeTrCBH I in L. starkeyi guided by signal peptides of L. starkeyi amylase or dextranase 2 gene, or Y. lipolytica protease gene. a Signal peptide of L. starkeyi amylase (spAMY); lanes 2–10, exoproteome produced by transformants integrated with plasmid pLS5-6. b Signal peptide of L. starkeyi dextranase 2 (spDEX2); lanes 1–10, exoproteome produced by transformants integrated with plasmid pLS8-2. c Signal peptide of Y. lipolytica protease (spPRO); lanes 1–10, exoproteome produced by transformants integrated with plasmid pLS4-1. For the samples in each of four panels (a–c), nCK, negative control, exoproteome produced by transformant integrated with empty vector; pCK, positive control, purified T. reesei CBH I protein. Culture supernatant was concentrated by 35×. Star indicates the transformants with the highest level of CBH I secretion in each subset of strains

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