Fig. 2

SDS-PAGE and western blot analysis of the overexpression of T. reesei eg2 gene in L. starkeyi guided by signal peptides derived from L. starkeyi amylase gene, dextranase 1 or 2 genes, or Y. lipolytica protease gene. a Signal peptide of L. starkeyi amylase (spAMY); lanes 1–10, exoproteome produced by transformants integrated with plasmid pLS2-2; star indicates the transformant with the highest level of EG II secretion in this subset of strains. b Signal peptide of L. starkeyi dextranase 1 (spDEX1); lanes 1–10, exoproteome produced by transformants integrated with plasmid pLS3-1. c Signal peptide of L. starkeyi dextranase 2 (spDEX2); lanes 1–10, exoproteome produced by transformants integrated with plasmid pLS7-1. d Signal peptide of Y. lipolytica protease (spPRO); lanes 1–4, exoproteome produced by transformants integrated with plasmid pLS1-3. For the samples in each of four panels (a–d), nCK, negative control, exproteome produced by transformant integrated with empty vector; pCK, positive control, purified T. reesei EGII protein. Culture supernatant was concentrated 2× for a and b, or 35× for c and d