Fig. 8

Parallel cultivations in shake flasks and laboratory-scale stirred tank reactors in batch and fed-batch operation mode with E. coli. a, c Batch cultivations with Wilms-MOPS-mineral medium (0.2 M MOPS), 20 g/L glucose, inoculation OD₆₀₀: 0.8. b, d Fed-batch cultivations with Wilms-MOPS-mineral medium (0.2 M MOPS), without initially provided glucose in culture broth, glucose in the reservoir of the fed-batch shake flask and in the feed bottle of the stirred tank reactor: 250 g/L, feeding of the fed-batch stirred tank reactor is started at the beginning of the experiment with a pumping rate of 4.2 mL/h, inoculation OD₆₀₀: 0.9; shake flask cultivation conditions: temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, culture broth volume: 10 mL, fed-batch reservoir filling volume: 2 mL, microfiltration membrane: Merck Millipore Isopore VCTP, membrane area: 11.34 mm²; stirred tank reactor conditions: temperature: 37 °C, aeration: 1 vvm, DOT controlled at 30% air saturation, reactor working volume: 1 L; strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP