Fig. 7
From: Systematic evaluation of characteristics of the membrane-based fed-batch shake flask
Online and offline characterization of cultivations with a dialysis membrane and a microfiltration membrane in E. coli fed-batch cultivations. a, b Oxygen transfer rate (OTR, solid line), glucose concentration in the reservoir (slanted triangles), glucose concentration in the culture broth (straight triangles), weight of reservoir content (filled squares). c, d Glucose concentration in the culture broth (half-filled triangles), pH (circles), osmolality (filled triangles) in the culture broth. e, f Acetate concentration (half-filled squares), cell dry weight (hexagons) and EcFbFP fluorescence (stars); cultivation conditions: Wilms-MOPS-mineral medium (0.2 M MOPS, 250 g/L glucose in the reservoir), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, culture broth volume: 10 mL, inoculation OD600: 0.7, reservoir filling volume: 2 mL, dialysis membrane: Reichelt (1) dialysis membrane, membrane area: 18.1 mm2, microfiltration membrane: Millipore VCTP Isopore, membrane area: 11.34 mm2; strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP