Fig. 6
From: Systematic evaluation of characteristics of the membrane-based fed-batch shake flask
Effect of molecular weight cut-off (MWCO), membrane type and material on oxygen transfer rate in E. coli fed-batch cultivations (duplicates). a Membrane tip type A (see Fig. 1b) with membrane area of 18.1 mm2 was used for the dialysis membranes with varying MWCO values [SpectraPor 25 kDa, SpectraPor 10 kDa, SpectraPor 1 kDa, Reichelt (1) dialysis membrane]. b Membrane tip type B (see Fig. 1b) with membrane area of 11.34 mm2 was used for membranes of varying materials (mixed cellulose ester (MCE), polycarbonate (PC), polyether sulfone (PES), polyethylene terephthalate (PET)); cultivation conditions: Wilms-MOPS-mineral medium (0.2 M MOPS, 250 g/L glucose in the reservoir), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, culture broth volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL; strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP