Fig. 5
From: Systematic evaluation of characteristics of the membrane-based fed-batch shake flask
Effects of membrane area and reservoir volume on oxygen transfer rate in E. coli fed-batch cultivations (duplicates). Variation of a membrane area. b Reservoir volume; cultivation conditions: Wilms-MOPS-mineral medium (0.2 M MOPS, 250 g/L glucose in the reservoir), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, culture broth volume: 10 mL, inoculation OD600: 0.5, 2 mL reservoir filling volume in a and varied reservoir filling volume in b, dialysis membranes: Reichelt (1) dialysis membrane, varied membrane area in a and 18.1 mm2 membrane area in b; strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP